連續(xù)培養(yǎng)
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連續(xù)培養(yǎng)又叫開放培養(yǎng)，是相對(duì)分批培養(yǎng)或密閉培養(yǎng)而言的。連續(xù)培養(yǎng)是采用有效的措施讓微生物在某特定的環(huán)境中保持旺盛生長(zhǎng)狀態(tài)的培養(yǎng)方法.微生物在一定條件下，如果不斷補(bǔ)充營(yíng)養(yǎng)物質(zhì)和排除有害的代謝產(chǎn)物，理論上，微生物都可保持恒定的對(duì)數(shù)生長(zhǎng).通常采用兩種方式：（1）恒濁培養(yǎng)，當(dāng)微生物在恒濁器中培養(yǎng)進(jìn)入對(duì)數(shù)期時(shí)，不斷從外界加入新鮮培養(yǎng)基，而同時(shí)又流出培養(yǎng)物，用光電池信號(hào)控制濁度，以維持恒定的細(xì)胞密度.（2）恒化培養(yǎng)，微生物在恒化器中培養(yǎng)，通過(guò)控制恒化器中微生物生長(zhǎng)所必需營(yíng)養(yǎng)物的濃度，以保證微生物持續(xù)生長(zhǎng).連續(xù)培養(yǎng)應(yīng)用于工業(yè)發(fā)酵中稱連續(xù)發(fā)酵，并已實(shí)際應(yīng)用，例如，連續(xù)發(fā)酵法生產(chǎn)酒精，半連續(xù)發(fā)酵法生產(chǎn)丙酮、丁醇等.GOOGLE英文翻譯:The adoption of effective measures to allow micro-organisms in a particular environment to maintain strong growth of the state of training methods. Microorganism under certain conditions, if the continuous supply of nutrients and removal of harmful metabolites, in theory, micro-organisms can remain constant growth of the logarithm of . commonly used in two ways: (1) constant turbid culture, when the micro-organisms cultivated at constant turbidity device into the right number of periods, and constantly adding fresh medium from the outside world, while at the same time out of cultures, the battery light signal control turbidity, in order to maintain a constant cell density (2). chemostat cultivation of microorganisms in the chemostat culture, by controlling the growth of microorganisms in chemostat necessary for the concentration of nutrients in order to ensure sustained growth of microorganisms. used in industrial fermentation in continuous culture said the continuous fermentation, and has practical applications, for example, continuous production of fermentation alcohol, semi-continuous fermentation production of acetone, butanol, and so on.
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